rdcjournalRoyal Dental College JournalE-Volume 8 Issue 1MultidisciplinaryEnglishJanuary- DecemberNNY2025119Periodontology Stem Cell-Based Periodontal Regeneration: Role of Periodontal Ligament Stem CellsEnglishY7072Harish KumarVV1EnglishNSanthoshVC2EnglishYSaumiyaGopal2EnglishYSarikaH3EnglishY10.67091/JRDC.2025.8116Periodontal disease leads to the irreversible damage of the structures that support teeth, such as the alveolar bone, periodontal ligament, and cementum. Traditional periodontal treatments are successful in managing infection and inflammation; however, achieving true regeneration of the lost tissues continues to pose a significant clinical challenge. Recent advancements in regenerative medicine have underscored the potential involvement of stem cells in the restoration of periodontal structures. Among the different populations of dental stem cells, periodontal ligament stem cells (PDLSCs) have garnered considerable attention due to their availability, ability to self-renew, potential for multi lineage differentiation, and essential role in maintaining periodontal health. This review article intends to provide an overview of the biological properties of PDLSCs, their regenerative capabilities, sources, mechanisms of action, and the current experimental and clinical evidence that supports their application in periodontal regeneration.EnglishBone graft; CD 105, Cytokines; Mesenchymal Stem cells; Periodontal ligamenthttps://www.rdcjournal.org/abstract?id=311References16191. Tomokiyo A, Wada N, Maeda H. Periodontal ligament stem cells: Regenerative potency in periodontium. Stem cells and development. 2019 Jul 1;28(15):974-85. doi:10.1089/scd.2019.0031. 2.Hu L, Liu Y, Wang S. Stem cell based tooth and periodontal regeneration. Oral Diseases. 2018 Jul;24(5):696-705. doi: 10.1111/odi.12703. 3.Cortellini P, Tonetti MS. Clinical concepts for regenerative therapy in intra bony defects. Periodontology 2000. 2015 Jun;68(1):282-307. doi: 10.1111/prd.12048. 4.Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, Brahim J et al. Investigation of multipotent postnatal stem cells from human periodontal ligament. The Lancet. 2004 Jul 10;364(9429):149-55. doi: 10.1016/S0140-6736(04)16627-0. 5.Bartold PM, Gronthos S. Standardization of criteria defining periodontal ligament stem cells. Journal of Dental Research. 2017 May;96(5):487-90. doi:10.1177/0022034517697653. 6.Ivanovski S, Gronthos S, Shi S, Bartold PM. Stem cells in the periodontal ligament. Oral Diseases. 2006 Jul;12(4):358-63. doi:10.1111/j.1601-0825.2006.01253.x. 7.Shi S, Bartold PM, Miura M, Seo BM, Robey PG, Gronthos S. The efficacy of mesenchymal stem cells to regenerate and repair dental structures. Orthodontics and;amp; craniofacial research. 2005 Aug;8(3):191-9. doi: 10.1111/j.1601-6343.2005.00331.x. 8.Bosshardt DD, Sculean A. Does periodontal tissue regeneration really work?. Periodontology 2000. 2009;51(1):208-19. doi: 10.1111/j.1600-0757.2009.00317.x. 9.Gronthos S, Mrozik K, Shi S, Bartold PM. Ovine periodontal ligament stem cells: isolation, characterization, and differentiation potential. Calcified tissue International. 2006 Nov;79(5):310-7. doi: 10.1007/s00223-006- 0040-4. 10.Trubiani O, Pizzicannella J, Caputi S, Marchisio M, Mazzon E, Paganelli R et al. Periodontal ligament stem cells: current knowledge and future perspectives. Stem Cells and Development. 2019 Aug 1;28(15):995- 1003. doi: 10.1089/scd.2019.0025. 11. Chen FM, Jin Y. Periodontal tissue engineering and regeneration: current approaches and expanding opportunities. Tissue Engineering Part B: Reviews. 2010 Apr;16(2):219-55. doi: 10.1089/ten.TEB.2009.0562. 12.Giannobile WV. Periodontal tissue engineering by growth factors. Bone. 1996 Jul 1;19(1):S23-37. doi: 10.1016/s8756- 3282(96)00127-5. 13.Nakahara T, Nakamura T, Kobayashi E, Kuremoto KI, Matsuno T, Tabata Y et al. In situ tissue engineering of periodontal tissues by seeding with periodontal ligament-derived cells. Tissue Engineering. 2004 Mar 1;10(3- 4):537-44. doi:10.1089/107632704323061898. 14.Ferrarotti F, Romano F, Gamba MN, Quirico A, Giraudi M, Audagna M et al. Human intrabony defect regeneration with micrografts containing dental pulp stem cells: a randomized controlled clinical trial. Journal of Clinical Periodontology. 2018 Jul;45(7):841-50. doi: 10.1111/jcpe.12931. 15.Bartold PM, Mcculloch CA, Narayanan AS, Pitaru S. Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology. Periodontology 2000. 2000 Oct 1;24(1).253-69. doi: 10.1034/j.1600-0757.2000.2240113.x.